The 101 Freeway will be closed from Glendale to Cahuenga boulevards Sunday through Wednesday nights, Caltrans officials said.
Both sides of the highway will be closed alternately from midnight to 5 a.m. while crews install loop detectors into the pavement, officials said.
Motorists are encouraged to consider alternate routes during the closure, which has been dubbed the Hollywood Jam Session.
Caltrans crews worked in the area earlier this month, and spokesman Patrick Chandler said it didn't cause too much disruption.
Loop detectors will track travel time and speed information that reflect real-time traffic conditions, according to Caltrans.
The data collected from the wire loops determine traffic volume by counting how many vehicles pass over a loop at any given time.
The installation is part of of the final stages of a $19.26 million project to repave the highway.
The project is funded by the State Highway Operation and Protection Program and is slated to be completed by the end of the year, officials said.